• Shivashankar Group
    Nuclear Mechanics & Genome Regulation Laboratory
  • Shivashankar Group
    Nuclear Mechanics & Genome Regulation Laboratory

Shivashankar Lab

Nuclear Mechanics & Genome Regulation Laboratory

The Nuclear Mechanics & Genome Regulation Laboratory

Our laboratory is interested in understanding the role of cell geometry on nuclear mechanics and genome regulation. Cells, under physiological conditions, acquire a number of well-defined morphologies. Alterations in their shape, by mechanical microenvironment and/or cytokine signals, have profound impact on tissue homeostasis. While cells undergo changes in shape, for example, during circulation, crawling, extrusion or transmigration; the extent and duration to which such shape changes occur would have critical roles in regulating nuclear function including gene expression. In this context, how cell shape modulation alters nuclear mechanical architecture and how it integrates with the 3D organization of chromosomes and transcription networks are rather unexplored. Understanding the biophysical design principles underlying such processes will have important implications in establishing mechano-chemical routes to cellular reprogramming and in developing biomarkers for early disease diagnosis. Centered on this theme, our ongoing studies are beginning to provide a quantitative framework to explore the coupling between cell geometry and genome regulation. For these studies, we employ a multidisciplinary approach combining microfabrication techniques to sculpt single cell geometry, high-resolution microscopy, genomics and theoretical modelling.


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NMGR

Mechanobiology Institute, Singapore

National University of Singapore

T-Lab, Level 10

5A Engineering Drive 1

Singapore 117411